matlab-based application  (MathWorks Inc)

Journal: bioRxiv

Article Title: Loss of signaling fidelity between astrocyte endfeet and adjacent cerebral arterioles in an amyloid mouse model of Alzheimer’s disease

doi: 10.1101/2025.01.24.634584

Figure Lengend Snippet: Spatial-temporal analyses of vascular-astrocyte endfeet activity by LAVA application . A . Cartoon illustration showing analyses of astrocyte endfeet pairings within individual arteriole segments (blue lines) during airpuff-induced dilations (white arrows). B , Two photon micrograph of a cerebral penetrating arteriole (red) and summated activity map of astrocyte Ca 2+ signals (green). C , Modeling and sampling of arteriole crosssections are used to produce linearized channel clips containing the vessel of interest (white) and perivascular spaces (Ca 2+ activity in green). Vascular tone is measured from cross sectional area measurements, as well as close capture of astrocyte endfeet (1 and 2). D , Same FOV shown in panel B assessed during whisker stimulation. Endfoot ROIs (1 and 2 pink) and vascular mask (white) determined from the linearized vessel model (panel C) are mapped onto raw channels. E , Measurements of vascular tone from linearlized model in panel C and Ca2+ levels recorded from each endfoot (1 and 2) in panel D are plotted to display temporal associations of field activity. F-G , Pie charts showing the functional make-up of endfeet-arteriole pairings for WT ( F ) and 5xFAD ( G ) mice. Red indicates arteriole dilations with a subsequent endfoot Ca 2+ response; Blue indicates arteriole dilations in the absence of an endfoot response; Yellow indicates endfoot Ca 2+ response in the absence of an arteriole dilation; Black indicates pairings with neither an arteriole dilation, nor an endfoot Ca 2+ response. H , Scatter plots showing the endfoot Ca 2+ response latency ( i.e. latency between initial stimulation induced vasodilation and subsequent endfoot Ca 2+ signals). I , Scatterplot shows evoked Ca 2+ transient amplitudes in arteriole-associated endfeet. J , Scatterplots show the proportional relationship between Ca 2+ transient amplitude in each endfoot relative to vessel cross sectional area in the immediately adjacent arteriole. In H and J , each plot symbol represents an individual endfoot-arteriole pairing. In I , each plot symbol represents an individual mouse. p values derived from two-tailed T tests.

Article Snippet: In depth analyses of vascular motion and spatially paired astrocyte calcium signaling were characterized using our custom MATLAB-based application called LAVA.

Techniques: Activity Assay, Sampling, Whisker Assay, Functional Assay, Derivative Assay, Two Tailed Test

Journal: bioRxiv

Article Title: Loss of signaling fidelity between astrocyte endfeet and adjacent cerebral arterioles in an amyloid mouse model of Alzheimer’s disease

doi: 10.1101/2025.01.24.634584

Figure Lengend Snippet: Spatial-temporal analyses of vascular-astrocyte endfeet activity by LAVA application . A . Cartoon illustration showing analyses of astrocyte endfeet pairings within individual arteriole segments (blue lines) during airpuff-induced dilations (white arrows). B , Two photon micrograph of a cerebral penetrating arteriole (red) and summated activity map of astrocyte Ca 2+ signals (green). C , Modeling and sampling of arteriole crosssections are used to produce linearized channel clips containing the vessel of interest (white) and perivascular spaces (Ca 2+ activity in green). Vascular tone is measured from cross sectional area measurements, as well as close capture of astrocyte endfeet (1 and 2). D , Same FOV shown in panel B assessed during whisker stimulation. Endfoot ROIs (1 and 2 pink) and vascular mask (white) determined from the linearized vessel model (panel C) are mapped onto raw channels. E , Measurements of vascular tone from linearlized model in panel C and Ca2+ levels recorded from each endfoot (1 and 2) in panel D are plotted to display temporal associations of field activity. F-G , Pie charts showing the functional make-up of endfeet-arteriole pairings for WT ( F ) and 5xFAD ( G ) mice. Red indicates arteriole dilations with a subsequent endfoot Ca 2+ response; Blue indicates arteriole dilations in the absence of an endfoot response; Yellow indicates endfoot Ca 2+ response in the absence of an arteriole dilation; Black indicates pairings with neither an arteriole dilation, nor an endfoot Ca 2+ response. H , Scatter plots showing the endfoot Ca 2+ response latency ( i.e. latency between initial stimulation induced vasodilation and subsequent endfoot Ca 2+ signals). I , Scatterplot shows evoked Ca 2+ transient amplitudes in arteriole-associated endfeet. J , Scatterplots show the proportional relationship between Ca 2+ transient amplitude in each endfoot relative to vessel cross sectional area in the immediately adjacent arteriole. In H and J , each plot symbol represents an individual endfoot-arteriole pairing. In I , each plot symbol represents an individual mouse. p values derived from two-tailed T tests.

Article Snippet: To investigate dynamic interactions specifically between cerebral arterioles (see ) and astrocyte endfeet we used a custom MATLAB-based application called LAVA (Movie S3 and ).

Techniques: Activity Assay, Sampling, Whisker Assay, Functional Assay, Derivative Assay, Two Tailed Test

Journal: Plants

Article Title: The Physicochemical and Rheological Properties of Green Banana Flour–Wheat Flour Bread Substitutions

doi: 10.3390/plants14020207

Figure Lengend Snippet: Cell structure analysis of 10% banana bread using MATLAB R2024a bread texture analyzer. ( a ): wheat flour bread; ( b ): Cavendish bread; ( c ): Ladyfinger bread; ( d ): Ducasse bread. Black-and-white photos: air pocket segmentation.

Article Snippet: The bread texture analyzer based on a MATLAB R2024a application was used to identify connected regions in a digital image of a bread sample and to calculate density description parameters [ ].

Techniques:

Journal: Plants

Article Title: The Physicochemical and Rheological Properties of Green Banana Flour–Wheat Flour Bread Substitutions

doi: 10.3390/plants14020207

Figure Lengend Snippet: Cell size segmentation and color coding of 10% banana bread using MATLAB R2024a bread texture analyzer. ( a ): wheat flour bread; ( b ): Cavendish bread; ( c ): Ladyfinger bread; ( d ): Ducasse bread.

Article Snippet: The bread texture analyzer based on a MATLAB R2024a application was used to identify connected regions in a digital image of a bread sample and to calculate density description parameters [ ].

Techniques: